Beacon: 20-0166: Alachlor in water by immunoassay, Microtiter Plate
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Official Method Name
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Alachlor Plate Kit |
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Current Revision
| 2010 |
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Media
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WATER |
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Instrumentation
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Immunoassay |
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Method Subcategory
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Organic |
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Method Source
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Citation
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Brief Method Summary
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The Beacon Alachlor Plate Kit uses polyclonal antibodies that bind both Alachlor and an Alachlor-enzyme conjugate. Alachlor in the sample competes with Alachlor-enzyme conjugate for a limited number of antibody binding sites. Antibodies, which bind Alachlor compounds, are immobilized to the inside of the test wells. In the assay procedure you will: •Add a sample containing Alachlor to a test well, followed by Alachlor-enzyme conjugate. The conjugate competes with any Alachlor in the sample for the same antibody binding sites. •Wash away any unbound molecules, after you incubate this mixture for 60 minutes. •Add clear substrate solution to each well. In the presence of bound Alachlor-enzyme conjugate, the substrate is converted to a blue compound. One enzyme molecule can convert many substrate molecules. Since the same number of antibody binding sites are available in every well, and each well receives the same number of Alachlor-enzyme conjugate molecules, a sample containing a low concentration of Alachlor allows the antibody to bind many Alachlor-enzyme conjugate molecules. The result is a dark blue solution. Conversely, a high concentration of Alachlor allows fewer Alachlor-enzyme conjugate molecules to be bound by the antibodies, resulting in a lighter blue solution.. |
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Scope and Application
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This method determines alachlor in water. |
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Applicable Concentration Range
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0.1 - 0.75 |
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Interferences
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Quality Control Requirements
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Sample Handling
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Maximum Holding Time
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Relative Cost
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Unknown |
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Sample Preparation Methods
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